Preliminary Course Programme

6th practical course in Biofilm Procedures
Copenhagen, Denmark, 24 & 25 October 2019

Thursday 24 October

12:00

Welcome and introduction to course, Thomas Bjarnsholt


12:15


Basic biofilm knowledge and in vitro biofilm models, Kasper Nørskov Kragh, Yijuan Xu


13:00


Biofilms in wounds, Klaus Kirketerp-Møller


14:00


Coffee and briefing on exercises (teams of 2 or 3 will be doing exercises together)


14:30


Practical sessions:

- Casting of semi solid gels with serum, blood and meat powder and preparation of wound model

- Application of antimicrobials (incl. dressings) to biofilm model for microscopy (BYO!)

- Sonication, serial dilution and spread plating biofilms for viable counts (premade by instructors, please send antimicrobials before the course if you want quantitative evaluation)


17:30- 18:00

MDR, claims and standardized methods, Helle Stendahl Andersen


18:00-22:00

Dinner

 

Friday 25 October

09:00

Morning coffee

09:15

The role of biofilms in chronic infections

Evaluation of in vitro and in vivo models

Culture based methods, microscopy, sequencing technologies, pros and cons

What can we learn from using transcriptomics and metabolomics technologies?

Thomas Bjarnsholt, Trine Rolighed Thomsen, Blaine Fritz, Kasper Thomsen, Yijuan Xu       

11:00

Practical sessions:

Staining of bacterial biofilms in wound section (PNA FISH and DAPI) and semi solid biofilms (Syto 9 and PI)

Quantitative evaluation of plates

Microscopy of stained wound and Semi-solid biofilms

Qualitative analysis of filter biofilm


12:00


Lunch


12.45


Continuation of practical sessions


14:15


Coffee Break


14:30


Discussion of results and sum up


15:00


End of course