Preliminary Course Programme

6th practical course in Biofilm Procedures
Copenhagen, Denmark, 1 & 2 November 2018

Thursday 1 November

12:00Welcome and introduction to course, Thomas Bjarnsholt
12:15Basic Biofilm Knowledge and in vitro biofilm models, Kasper Nørskov Kragh
13:00Biofilms in wounds, Klaus Kirketerp-Møller
14:00Coffee and briefing on exercises (teams of 2 or 3 will be doing exercises together)
14:30Practical sessions:
  • Casting of semi solid gels with serum, blood and meat powder and preparation of wound model
  • Application of Antimicrobials (incl. dressings) to biofilm model for microscopy (BYO!)
  • Sonication, serial dilution and spread plating biofilms for viable counts (premade by instructors,

please send antimicrobials before the course if you want quantitative evaluation)

  • Production of alginate beads


Friday 2 November

09:30Morning coffee

The role of biofilms in chronic infections, Thomas Bjarnsholt         
In vivo biofilm models and novel treatment strategies, Maria Alhede

10:45Practical sessions:
  • Staining of bacterial biofilms in wound section (PNA FISH and DAPI) and semi solid biofilms (Syto 9 and PI)
  • Quantitative evaluation of plates
  • Microscopy of stained wound, Semi-solid biofilms and Alginate beads
  • Qualitative analysis of filter biofilm
12.45Continuation of practical sessions
14:15Coffee Break
14:30Discussion of results and sum up
15:00End of course